Regulatory

Part:BBa_J100531:Design

Designed by: Kaitlin Cvijanovich, Alexandra Johansen, Yusef Thomas, Douglas Deutsch   Group: Campbell M Lab   (2020-02-06)


A constitutive promoter that has the tet-kil gene but not the cI857 repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter sequence was originally 67 base pairs so we cut the last eleven base pairs of the sequence in order to fit the golden gate assembly sticky ends.


Source

https://www.sciencedirect.com/science/article/pii/S0168165619308946

References